Record keeping

• A simple electronic lab notebook

• Data management

Molecular cloning

• Gibson assembly

• repp-assisted plasmid design

• Golden gate assembly

• Freezing and streaking bacteria

• Miniprep (regular)

• Gel purification

• PCR amplification

• Commonly used E. coli genotypes

Cell and organ culture

• Useful cell numbers in various culture formats

• Isolation and culture of mouse embryonic salivary glands

• Isolation and culture of salivary gland epithelial buds

Lentivirus packaging and transduction

• Lentivirus packaging

• Lentivirus transduction of salivary gland epithelial buds

Genome engineering

• Guide RNA design for gene knockout

Immunostaining

• Whole-mount immunostaining for mouse embryonic organs or 3D spheroid cultures

Single-molecule RNA fluorescence in situ hybridization (smFISH)

• smFISH for tissue culture cells

• smFISH for whole-mount mouse embryonic organs

Protein biochemistry

• Microtubule co-sedimentation from worm exacts