Different cell lines can have different sizes and growth properties that will affect the accuracy of this estimate, but it can serve as a starting point to guide experimental design.

1. Cell number for indicated confluence levels

Culture format	Area (cm2)	100%	30%	20%
10-cm dish	78	1×107	3×106	2×106
T75 flask	75	1×107	3×106	2×106
35-mm dish	9.6	1.2×106	3.6×105	2.4×105
6-well plate	9.6	1.2×106	3.6×105	2.4×105
12-well plate	3.5	4.3×105	1.3×105	8.6×104
24-well plate	1.9	2.4×105	7.2×104	4.8×104
96-well plate	0.32	4×104	1.2×104	8×103
8-well ibidi chamber	1	1.3×105	3.9×104	2.5×104

2. Cheat sheet for 8-well ibidi chamber seeding

Cells / mL	µL for 10%	20%	30%	50%	100%
5×105	25	50	75	125	250
6×105	21	42	63	104	208
7×105	18	36	54	89	179
8×105	16	31	47	78	156
9×105	14	28	42	69	139
1×106	13	25	38	63	125
1.1×106	11	23	34	57	114
1.2×106	10	21	31	52	104
1.3×106	10	19	29	48	96
1.4×106	8.9	18	27	45	89
1.5×106	8.3	17	25	42	83
1.6×106	7.8	16	23	39	78
1.7×106	7.4	15	22	37	74
1.8×106	6.9	14	21	35	69
1.9×106	6.6	13	20	33	66
2×106	6.3	13	19	31	63